Integrated Microfluidic Lectin Barcode Platform for High-Performance Focused Glycomic Profiling.

ABSTRACT: Protein glycosylation is one of the key processes that play essential roles in biological functions and dysfunctions. However, progress in glycomics has considerably lagged behind genomics and proteomics, due in part to the enormous challenges in analysis of glycans. Here we present a new integrated and automated microfluidic lectin barcode platform to substantially improve the performance of lectin array for focused glycomic profiling. The chip design and flow control were optimized to promote the lectin-glycan binding kinetics and speed of lectin microarray. Moreover, we established an on-chip lectin assay which employs a very simple blocking method to effectively suppress the undesired background due to lectin binding of antibodies. Using this technology, we demonstrated focused differe

SUBMITTER: Shang Y 

PROVIDER: S-EPMC4735825 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature