Phylogenetic and Transcripts Profiling of Glucose Sensing Related Genes in Candida glabrata.

ABSTRACT:

Background

The sensing mechanism of glucose in Saccharomyces cerevisiae is well studied. However, such information is scarcely found in other yeast species such as Candida glabrata.

Objectives

This study aimed to identify the glucose sensing pathway related genes of C. glabrata and to analyze the regulation pattern of these genes in response to different surrounding glucose concentrations through the quantitative real time polymerase chain reaction (qRT-PCR).

Materials and methods

Phylogenetic analysis was carried out on predicted amino acid sequences of C. glabrata and S. cerevisiae to compare their degree of similarity. In addition, the growth of C. glabrata in response to different amounts of glucose (0%, 0.01%, 0.1%, 1% and 2%) was evaluated via the spot dilution

SUBMITTER: Ng TS 

PROVIDER: S-EPMC4735840 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature