A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard.

ABSTRACT:

Background

The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the construction of transcriptional gene circuits, many of which depend on the technical ability to compose and transfer complex synthetic instructions into the plant cell. The engineering principles of standardization and modularity applied to DNA cloning are impacting plant genetic engineering, by increasing multigene assembly efficiency and by fostering the exchange of well-defined physical DNA parts with precise functional information.

Results

Here we describe the adaptation of the RNA-guided Cas9 system to GoldenBraid (GB), a modular DNA construction framework being in

SUBMITTER: Vazquez-Vilar M 

PROVIDER: S-EPMC4736081 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature