Different subtype strains of Akkermansia muciniphila abundantly colonize in southern China.

ABSTRACT:

Aim

This study investigates the colonization rate of Akkermansia muciniphila in the gastrointestinal tracts of people living in southern China and applies a modified method for the isolation and subtyping of A. muciniphila strains from faecal samples.

Methods and results

Fresh faecal samples were collected and bacterial DNA was extracted from these samples for real-time PCR analysis. Strains were separated using a culture-dependent sPCR-directed method and classified using an enterobacterial repetitive intergenic consensus (ERIC-PCR) DNA fingerprinting method. The colonization rate for the sample population from southern China was 51·74%. We isolated 22 strains from human faeces. The results revealed that all strains were identifiable as A. muciniphila with 99-100% identity

SUBMITTER: Guo X 

PROVIDER: S-EPMC4736461 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature