Proteomic analysis of integrin-associated complexes from mesenchymal stem cells.

ABSTRACT:

Purpose

Multipotent mesenchymal stem cells (MSCs) have the capability to differentiate down adipocyte, osteocyte and chondrocyte lineages and as such offer a range of potential therapeutic applications. The composition and stiffness of the extracellular matrix (ECM) environment that surrounds cells dictates their transcriptional programme, thereby affecting stem cell lineage decision-making. Cells sense force via linkages between themselves and their microenvironment, and this is transmitted by integrin receptors and associated adhesion signalling complexes. To identify regulators of MSC force sensing, we sought to catalogue MSC integrin-associated adhesion complex composition.

Experimental design

Adhesion complexes formed by MSCs plated on the ECM ligand fibronectin were is

SUBMITTER: Ajeian JN 

PROVIDER: S-EPMC4737105 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature