Unknown

Dataset Information

0

Aneuploidy-dependent massive deregulation of the cellular transcriptome and apparent divergence of the Wnt/beta-catenin signaling pathway in human rectal carcinomas.


ABSTRACT: To identify genetic alterations underlying rectal carcinogenesis, we used global gene expression profiling of a series of 17 locally advanced rectal adenocarcinomas and 20 normal rectal mucosa biopsies on oligonucleotide arrays. A total of 351 genes were differentially expressed (P < 1.0e-7) between normal rectal mucosa and rectal carcinomas, 77 genes had a >5-fold difference, and 85 genes always had at least a 2-fold change in all of the matched samples. Twelve genes satisfied all three of these criteria. Altered expression of genes such as PTGS2 (COX-2), WNT1, TGFB1, VEGF, and MYC was confirmed, whereas our data for other genes, like PPARD and LEF1, were inconsistent with previous reports. In addition, we found deregulated expression of many genes whose involvement in rectal carcinogenesis has not been reported. By mapping the genomic imbalances in the tumors using comparative genomic hybridization, we could show that DNA copy number gains of recurrently aneuploid chromosome arms 7p, 8q, 13q, 18q, 20p, and 20q correlated significantly with their average chromosome arm expression profile. Taken together, our results show that both the high-level, significant transcriptional deregulation of specific genes and general modification of the average transcriptional activity of genes residing on aneuploid chromosomes coexist in rectal adenocarcinomas.

SUBMITTER: Grade M 

PROVIDER: S-EPMC4737482 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Aneuploidy-dependent massive deregulation of the cellular transcriptome and apparent divergence of the Wnt/beta-catenin signaling pathway in human rectal carcinomas.

Grade Marian M   Ghadimi B Michael BM   Varma Sudhir S   Simon Richard R   Wangsa Danny D   Barenboim-Stapleton Linda L   Liersch Torsten T   Becker Heinz H   Ried Thomas T   Difilippantonio Michael J MJ  

Cancer research 20060101 1


To identify genetic alterations underlying rectal carcinogenesis, we used global gene expression profiling of a series of 17 locally advanced rectal adenocarcinomas and 20 normal rectal mucosa biopsies on oligonucleotide arrays. A total of 351 genes were differentially expressed (P < 1.0e-7) between normal rectal mucosa and rectal carcinomas, 77 genes had a >5-fold difference, and 85 genes always had at least a 2-fold change in all of the matched samples. Twelve genes satisfied all three of thes  ...[more]

Similar Datasets

| S-EPMC4721580 | biostudies-literature
2011-03-17 | GSE20970 | GEO
2011-03-17 | E-GEOD-20970 | biostudies-arrayexpress
| S-EPMC3643417 | biostudies-literature
| S-EPMC10368384 | biostudies-literature
2017-10-30 | GSE79330 | GEO
2017-10-30 | GSE78250 | GEO
2017-10-30 | GSE79328 | GEO
| S-EPMC3227636 | biostudies-literature