ADAR-mediated RNA editing suppresses sleep by acting as a brake on glutamatergic synaptic plasticity.

ABSTRACT: It has been postulated that synaptic potentiation during waking is offset by a homoeostatic reduction in net synaptic strength during sleep. However, molecular mechanisms to support such a process are lacking. Here we demonstrate that deficiencies in the RNA-editing gene Adar increase sleep due to synaptic dysfunction in glutamatergic neurons in Drosophila. Specifically, the vesicular glutamate transporter is upregulated, leading to over-activation of NMDA receptors, and the reserve pool of glutamatergic synaptic vesicles is selectively expanded in Adar mutants. Collectively these changes lead to sustained neurotransmitter release under conditions that would otherwise result in synaptic depression. We propose that a shift in the balance from synaptic depression towards synaptic potentiatio

SUBMITTER: Robinson JE 

PROVIDER: S-EPMC4737855 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature