Functional environmental proteomics: elucidating the role of a c-type cytochrome abundant during uranium bioremediation.

ABSTRACT: Studies with pure cultures of dissimilatory metal-reducing microorganisms have demonstrated that outer-surface c-type cytochromes are important electron transfer agents for the reduction of metals, but previous environmental proteomic studies have typically not recovered cytochrome sequences from subsurface environments in which metal reduction is important. Gel-separation, heme-staining and mass spectrometry of proteins in groundwater from in situ uranium bioremediation experiments identified a putative c-type cytochrome, designated Geobacter subsurface c-type cytochrome A (GscA), encoded within the genome of strain M18, a Geobacter isolate previously recovered from the site. Homologs of GscA were identified in the genomes of other Geobacter isolates in the phylogenetic cluster known as s

SUBMITTER: Yun J 

PROVIDER: S-EPMC4737924 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature