Targeting Cancer Stem Cells in Castration-Resistant Prostate Cancer.

ABSTRACT:

Purpose

Clinical evidence suggests increased cancer stem cells (CSCs) in a tumor mass may contribute to the failure of conventional therapies because CSCs seem to be more resistant than differentiated tumor cells. Thus, unveiling the mechanism regulating CSCs and candidate target molecules will provide new strategy to cure the patients.

Experimental design

The stem-like cell properties were determined by a prostasphere assay and dye exclusion assay. To find critical stem cell marker and reveal regulation mechanism, basic biochemical and molecular biologic methods, such as quantitative real-time PCR, Western blot, reporter gene assay, and chromatin immunoprecipitation assay, were used. In addition, to determine the effect of combination therapy targeting both CSCs and its pro

SUBMITTER: Yun EJ 

PROVIDER: S-EPMC4738061 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature