Exploiting the CRISPR/Cas9 System for Targeted Genome Mutagenesis in Petunia.

ABSTRACT: Recently, CRISPR/Cas9 technology has emerged as a powerful approach for targeted genome modification in eukaryotic organisms from yeast to human cell lines. Its successful application in several plant species promises enormous potential for basic and applied plant research. However, extensive studies are still needed to assess this system in other important plant species, to broaden its fields of application and to improve methods. Here we showed that the CRISPR/Cas9 system is efficient in petunia (Petunia hybrid), an important ornamental plant and a model for comparative research. When PDS was used as target gene, transgenic shoot lines with albino phenotype accounted for 55.6%-87.5% of the total regenerated T0 Basta-resistant lines. A homozygous deletion close to 1 kb in length can be re

SUBMITTER: Zhang B 

PROVIDER: S-EPMC4738242 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature