Genetic Evolution during the development of an attenuated EIAV vaccine.

ABSTRACT:

Background

The equine infectious anemia virus (EIAV) vaccine is the only attenuated lentiviral vaccine applied on a large scale that has been shown to be effective in controlling the prevalence of EIA in China. This vaccine was developed by successive passaging of a field-isolated virulent strain in different hosts and cultivated cells. To explore the molecular basis for the phenotype alteration of this vaccine strain, we systematically analyzed its genomic evolution during vaccine development.

Results

Sequence analysis revealed that the genetic distance between the wild-type strain and six representative strains isolated from key development stages gradually increased with the number of passages. Env gene, but not gag and pol, showed a clear evolutionary flow similar to tha

SUBMITTER: Wang XF 

PROVIDER: S-EPMC4738788 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature