Immunolocalization and Distribution of Rubella Antigen in Fatal Congenital Rubella Syndrome.

ABSTRACT:

Background

An estimated 100,000 cases of congenital rubella syndrome (CRS) occur worldwide each year. The reported mortality rate for infants with CRS is up to 33%. The cellular mechanisms responsible for the multiple congenital defects in CRS are presently unknown. Here we identify cell types positive for rubella virus (RV) in CRS infants.

Methods

Cells and organs involved in RV replication were identified in paraffin-embedded autopsy tissues from three fatal case-patients by histopathologic examination and immunohistochemical (IHC) staining using a rabbit polyclonal RV antibody. Normal rabbit antisera and RV antisera preabsorbed with highly purified RV served as negative controls.

Results

RV antigen was found in interstitial fibroblasts in the heart, adventitial fib

SUBMITTER: Lazar M 

PROVIDER: S-EPMC4739417 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature