Differential T-helper cell polarization after allergen-specific stimulation of autologous dendritic cells in polysensitized allergic patients.

ABSTRACT:

Background

Dendritic cells (DCs) play an important role in the induction and regulation of adaptive immune responses by polarizing T-helper (Th) cells. In allergic disease this response is dominated by Th2 cells. It is still unclear whether the activation of Th cells by DCs in atopic individuals is allergen specific.

Methods

Monocyte-derived DCs (MoDCs) obtained from polysensitized patients were stimulated with purified Bet v 1, Phl p 5 and Act d 10, and the surface marker expression was analysed. Proliferation and cytokine profiles of autologous naïve CD4+ T cells co-cultured with allergen-pulsed MoDCs were assessed.

Results

The addition of either Bet v 1 or Phl p 5 did not further increase the expression of surface markers from matured MoDCs in all study groups. In

SUBMITTER: Ashjaei K 

PROVIDER: S-EPMC4739505 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature