Identification of Serum Biomarkers for Gastric Cancer Diagnosis Using a Human Proteome Microarray.

ABSTRACT: We aimed to globally discover serum biomarkers for diagnosis of gastric cancer (GC). GC serum autoantibodies were discovered and validated using serum samples from independent patient cohorts encompassing 1,401 participants divided into three groups, i.e. healthy, GC patients, and GC-related disease group. To discover biomarkers for GC, the human proteome microarray was first applied to screen specific autoantibodies in a total of 87 serum samples from GC patients and healthy controls. Potential biomarkers were identified via a statistical analysis protocol. Targeted protein microarrays with only the potential biomarkers were constructed and used to validate the candidate biomarkers using 914 samples. To provide further validation, the abundance of autoantibodies specific to the biomarker

SUBMITTER: Yang L 

PROVIDER: S-EPMC4739676 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature