Transcellular movement of hydroxyurea is mediated by specific solute carrier transporters.

ABSTRACT:

Objective

Hydroxyurea has proven laboratory and clinical therapeutic benefits for sickle cell anemia and other diseases, yet many questions remain about its in vivo pharmacokinetic and pharmacodynamic profiles. Previous reports suggest that hydroxyurea passively diffuses across cells, but its observed rapid absorption and distribution are more consistent with facilitated or active transport. We investigated the potential role of solute carrier (SLC) transporters in cellular uptake and accumulation of hydroxyurea.

Materials and methods

Passive diffusion of hydroxyurea across cell membranes was determined using the parallel artificial membrane permeability assay. SLC transporter screens were conducted using in vitro intracellular drug accumulation and transcellular transport a

SUBMITTER: Walker AL 

PROVIDER: S-EPMC4739778 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature