Widespread neuronal injury in a model of cholinergic status epilepticus in postnatal day 7 rat pups.

ABSTRACT:

Objective

Status Epilepticus (SE) is common in neonates and infants, and is associated with neuronal injury and adverse developmental outcomes. However, the role of SE in this injury is uncertain. Until now, we have lacked an animal model in which seizures result in neuronal injury in rodent models at ages below postnatal day 12 (P12) unless seizures are combined with inflammatory stressors.

Methods

We induced SE with high-dose lithium and pilocarpine in P7 rats, which are developmentally close to human neonates. Several EEG measures and O2 saturation were recorded during the 6h following initiation of SE. We assessed neuronal injury at 6 and 24h post-SE onset using Fluoro-Jade B staining (FJB) and caspase-3a immunoreactivity (IR).

Results

EEGs showed continuous polys

SUBMITTER: Torolira D 

PROVIDER: S-EPMC4740206 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature