Crimean-Congo Hemorrhagic Fever Virus Gn Bioinformatic Analysis and Construction of a Recombinant Bacmid in Order to Express Gn by Baculovirus Expression System.

ABSTRACT:

Background

Crimean-Congo hemorrhagic fever virus (CCHFV) is a member of the nairovirus, a genus in the Bunyaviridae family, which causes a life threatening disease in human. Currently, there is no vaccine against CCHFV and detailed structural analysis of CCHFV proteins remains undefined. The CCHFV M RNA segment encodes two viral surface glycoproteins known as Gn and Gc. Viral glycoproteins can be considered as key targets for vaccine development.

Objectives

The current study aimed to investigate structural bioinformatics of CCHFV Gn protein and design a construct to make a recombinant bacmid to express by baculovirus system.

Materials and methods

To express the Gn protein in insect cells that can be used as antigen in animal model vaccine studies. Bioinformatic analys

SUBMITTER: Rahpeyma M 

PROVIDER: S-EPMC4740762 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature