Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound.

ABSTRACT: Controlling cell functions for research and therapeutic purposes may open new strategies for the treatment of many diseases. An efficient and safe introduction of membrane impermeable molecules into target cells will provide versatile means to modulate cell fate. We introduce a new transfection technique that utilizes high frequency ultrasound without any contrast agents such as microbubbles, bringing a single-cell level targeting and size-dependent intracellular delivery of macromolecules. The transfection apparatus consists of an ultrasonic transducer with the center frequency of over 150 MHz and an epi-fluorescence microscope, entitled acoustic-transfection system. Acoustic pulses, emitted from an ultrasonic transducer, perturb the lipid bilayer of the cell membrane of a targeted single

SUBMITTER: Yoon S 

PROVIDER: S-EPMC4740885 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature