Transgelin increases metastatic potential of colorectal cancer cells in vivo and alters expression of genes involved in cell motility.

ABSTRACT:

Background

Transgelin is an actin-binding protein that promotes motility in normal cells. Although the role of transgelin in cancer is controversial, a number of studies have shown that elevated levels correlate with aggressive tumor behavior, advanced stage, and poor prognosis. Here we sought to determine the role of transgelin more directly by determining whether experimental manipulation of transgelin levels in colorectal cancer (CRC) cells led to changes in metastatic potential in vivo.

Methods

Isogenic CRC cell lines that differ in transgelin expression were characterized using in vitro assays of growth and invasiveness and a mouse tail vein assay of experimental metastasis. Downstream effects of transgelin overexpression were investigated by gene expression profiling a

SUBMITTER: Zhou HM 

PROVIDER: S-EPMC4741053 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature