EGFRvIII does not affect radiosensitivity with or without gefitinib treatment in glioblastoma cells.

ABSTRACT:

Background

Glioblastomas (GBM) are often characterized by an elevated expression of the epidermal growth factor receptor variant III (EGFRvIII). We used GBM cell lines with native EGFRvIII expression to determine whether this EGFR variant affects radiosensitivity with or without EGFR targeting.

Methods

Experiments were performed with GBM cell lines lacking (LN229, U87MG, U251, CAS-1) or endogenously expressing EGFRvIII (BS153, DKMG). The two latter cell lines were also used to establish sublines with a low (-) or a high proportion (+) of cells expressing EGFRvIII. EGFR signaling and the cell cycle were analyzed using Western blot and flow cytometry; cell survival was assessed by colony forming assay and double-strand break repair capacity by immunofluorescence.

Results

SUBMITTER: Struve N 

PROVIDER: S-EPMC4741808 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature