Ageing is associated with molecular signatures of inflammation and type 2 diabetes in rat pancreatic islets.

ABSTRACT:

Aims/hypothesis

Ageing is a major risk factor for development of metabolic diseases such as type 2 diabetes. Identification of the mechanisms underlying this association could help to elucidate the relationship between age-associated progressive loss of metabolic health and development of type 2 diabetes. We aimed to determine molecular signatures during ageing in the endocrine pancreas.

Methods

Global gene transcription was measured in pancreatic islets isolated from young and old rats by Ilumina BeadChip arrays. Promoter DNA methylation was measured by Sequenom MassArray in 46 genes that showed differential expression with age, and correlations with expression were established. Alterations in morphological and cellular processes with age were determined by immunohistochemi

SUBMITTER: Sandovici I 

PROVIDER: S-EPMC4742511 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature