In silico analysis of Brucella abortus Omp2b and in vitro expression of SOmp2b.

ABSTRACT:

Purpose

At present, there is no vaccine available for the prevention of human brucellosis. Brucella outer membrane protein 2b (Omp2b) is a 36 kD porin existed in common Brucella pathogens and it is considered as priority antigen for designing a new subunit vaccine.

Materials and methods

In the current study, we aimed to predict and analyze the secondary and tertiary structures of the Brucella abortus Omp2b protein, and to predict T-cell and B-cell epitopes with the help of bioinformatics tools. Subsequently, cloning and expression of the short form of Omp2b (SOmp2b) was performed using pET28a expression vector and Escherichia coli BL21 host, respectively. The recombinant SOmp2b (rSOmp2b) was purified with Ni-NTA column.

Results

The recombinant protein was successfully

SUBMITTER: Golshani M 

PROVIDER: S-EPMC4742603 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature