Identification of the Glycosaminoglycan Binding Site of Interleukin-10 by NMR Spectroscopy.

ABSTRACT: The biological function of interleukin-10 (IL-10), a pleiotropic cytokine with an essential role in inflammatory processes, is known to be affected by glycosaminoglycans (GAGs). GAGs are highly negatively charged polysaccharides and integral components of the extracellular matrix with important functions in the biology of many growth factors and cytokines. The molecular mechanism of the IL-10/GAG interaction is unclear. In particular, experimental evidence about IL-10/GAG binding sites is lacking, despite its importance for understanding the biological role of the interaction. Here, we report the experimental determination of a GAG binding site of IL-10. Although no co-crystal structure of the IL-10·GAG complex could be obtained, its structural characterization was possible by NMR spectros

SUBMITTER: Kunze G 

PROVIDER: S-EPMC4742770 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature