A monoclonal antibody against KCNK9 K(+) channel extracellular domain inhibits tumour growth and metastasis.

ABSTRACT: Two-pore domain potassium (K2P) channels act to maintain cell resting membrane potential--a prerequisite for many biological processes. KCNK9, a member of K2P family, is implicated in cancer, owing to its overexpression in human tumours and its ability to promote neoplastic cell survival and growth. However, KCNK9's underlying contributions to malignancy remain elusive due to the absence of specific modulators. Here we describe the development of monoclonal antibodies against the KCNK9 extracellular domain and their functional effects. We show that one antibody (Y4) with the highest affinity binding induces channel internalization. The addition of Y4 to KCNK9-expressing carcinoma cells reduces cell viability and increases cell death. Systemic administration of Y4 effectively inhibits growt

SUBMITTER: Sun H 

PROVIDER: S-EPMC4742836 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature