SciReptor: analysis of single-cell level immunoglobulin repertoires.

ABSTRACT:

Background

The sequencing of immunoglobulin (Ig) transcripts from single B cells yields essential information about Ig heavy:light chain pairing, which is lost in conventional bulk sequencing experiments. The previously limited throughput of single-cell approaches has recently been overcome by the introduction of multiple next-generation sequencing (NGS)-based platforms. Furthermore, single-cell techniques allow the assignment of additional data types (e.g. cell surface marker expression), which are crucial for biological interpretation. However, the currently available computational tools are not designed to handle single-cell data and do not provide integral solutions for linking of sequence data to other biological data.

Results

Here we introduce sciReptor, a flexible too

SUBMITTER: Imkeller K 

PROVIDER: S-EPMC4743164 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature