Kruppel-like factor 4 expression in oral carcinoma cells and hypermethylation at the gene promoter.

ABSTRACT:

Background

Krüppel-like factor 4 (KLF4) is a transcription factor regulating proliferation-differentiation balance of epithelium, and down-regulated in less-differentiated and advanced oral carcinomas. Although the expression is inactivated by the promoter hypermethylation in malignant tumor cells, it remains unknown in oral carcinoma cells.

Methods

Genomic DNA isolated from nine different oral carcinoma cell lines and a normal keratinocyte line was treated with sodium bisulfite, and methylation at KLF4 gene promoter was determined by PCR direct-sequence analysis. KLF4 expression in cells cultured with or without demethylation reagent was monitored by quantitative real-time PCR and immunoblot.

Results

A 237-bp promoter region spanning - 718 and - 482 of KLF4 gene was

SUBMITTER: Yamaguchi A 

PROVIDER: S-EPMC4743192 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature