Revisiting Cardiac Cellular Composition.

ABSTRACT:

Rationale

Accurate knowledge of the cellular composition of the heart is essential to fully understand the changes that occur during pathogenesis and to devise strategies for tissue engineering and regeneration.

Objective

To examine the relative frequency of cardiac endothelial cells, hematopoietic-derived cells, and fibroblasts in the mouse and human heart.

Methods and results

Using a combination of genetic tools and cellular markers, we examined the occurrence of the most prominent cell types in the adult mouse heart. Immunohistochemistry revealed that endothelial cells constitute >60%, hematopoietic-derived cells 5% to 10%, and fibroblasts <20% of the nonmyocytes in the heart. A refined cell isolation protocol and an improved flow cytometry approach provided an ind

SUBMITTER: Pinto AR 

PROVIDER: S-EPMC4744092 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature