Cryopreservation and recovery of human endometrial epithelial cells with high viability, purity, and functional fidelity.

ABSTRACT:

Objective

To develop a protocol for cryopreservation and recovery of human endometrial epithelial cells (eECs) retaining molecular and functional characteristics of endometrial epithelium in vivo.

Design

In vitro study using human endometrial cells.

Setting

University research laboratory.

Patient(s)

Endometrial biopsies were obtained from premenopausal women undergoing benign gynecologic procedures.

Intervention(s)

Primary eECs were cryopreserved in 1% fetal bovine serum/10% dimethylsulfoxide in Defined Keratinocyte Serum-Free Medium (KSFM). Recovered cells were observed for endometrial stromal fibroblast (eSF) contamination and subsequently evaluated for morphology, gene expression, and functional characteristics of freshly cultured eECs and in vivo end

SUBMITTER: Chen JC 

PROVIDER: S-EPMC4744114 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature