Hyperglycaemia attenuates in vivo reprogramming of pancreatic exocrine cells to beta cells in mice.

ABSTRACT:

Aims/hypothesis

Reprogramming of pancreatic exocrine to insulin-producing cells by viral delivery of the genes encoding transcription factors neurogenin-3 (Ngn3), pancreas/duodenum homeobox protein 1 (Pdx1) and MafA is an efficient method for reversing diabetes in murine models. The variables that modulate reprogramming success are currently ill-defined.

Methods

Here, we assess the impact of glycaemia on in vivo reprogramming in a mouse model of streptozotocin-induced beta cell ablation, using subsequent islet transplantation or insulin pellet implantation for creation of groups with differing levels of glycaemia before viral delivery of transcription factors.

Results

We observed that hyperglycaemia significantly impaired reprogramming of exocrine to insulin-producing

SUBMITTER: Cavelti-Weder C 

PROVIDER: S-EPMC4744133 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature