Subacute limb ischemia induces skeletal muscle injury in genetically susceptible mice independent of vascular density.

ABSTRACT:

Objective

The primary preclinical model of peripheral artery disease, which involves acute limb ischemia (ALI), can result in appreciable muscle injury that is attributed to the acuity of the ischemic injury. A less acute model of murine limb ischemia using ameroid constrictors (ACs) has been developed in an attempt to mimic the chronic nature of human disease. However, there is currently little understanding of how genetics influence muscle injury following subacute arterial occlusion in the mouse.

Methods

We investigated the influence of mouse genetics on skeletal muscle tissue survival, blood flow, and vascular density by subjecting two different mouse strains, C57BL/6 (BL6) and BALB/c, to ALI or subacute limb ischemia using single (1AC) or double (2AC) AC placement on th

SUBMITTER: McClung JM 

PROVIDER: S-EPMC4744137 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature