Automated Ex Situ Assays of Amyloid Formation on a Microfluidic Platform.

ABSTRACT: Increasingly prevalent neurodegenerative diseases are associated with the formation of nanoscale amyloid aggregates from normally soluble peptides and proteins. A widely used strategy for following the aggregation process and defining its kinetics involves the use of extrinsic dyes that undergo a spectral shift when bound to β-sheet-rich aggregates. An attractive route to carry out such studies is to perform ex situ assays, where the dye molecules are not present in the reaction mixture, but instead are only introduced into aliquots taken from the reaction at regular time intervals to avoid the possibility that the dye molecules interfere with the aggregation process. However, such ex situ measurements are time-consuming to perform, require large sample volumes, and do not provide for real

SUBMITTER: Saar KL 

PROVIDER: S-EPMC4744157 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature