Effects of heat-treatment on the stability and composition of metabolomic extracts from the earthworm Eisenia fetida.
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ABSTRACT:
SUBMITTER: Schock TB
PROVIDER: S-EPMC4744258 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
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Effects of heat-treatment on the stability and composition of metabolomic extracts from the earthworm <i>Eisenia fetida</i>.
Schock Tracey B TB Strickland Sheri S Steele Edna J EJ Bearden Daniel W DW
Metabolomics : Official journal of the Metabolomic Society 20160205
Environmental metabolomics studies employing earthworms as sentinels for soil contamination are numerous, but the instability of the metabolite extracts from these organisms has been minimally addressed. This study evaluated the efficacy of adding a heat-treatment step in two commonly used extraction protocols (Bligh and Dyer and D<sub>2</sub>O phosphate buffer) as a pre-analytical stabilization method. The resulting metabolic profiles of <i>Eisenia fetida</i> were assessed using principal compo ...[more]
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