Genome ARTIST: a robust, high-accuracy aligner tool for mapping transposon insertions and self-insertions.

ABSTRACT:

Background

A critical topic of insertional mutagenesis experiments performed on model organisms is mapping the hits of artificial transposons (ATs) at nucleotide level accuracy. Mapping errors may occur when sequencing artifacts or mutations as single nucleotide polymorphisms (SNPs) and small indels are present very close to the junction between a genomic sequence and a transposon inverted repeat (TIR). Another particular item of insertional mutagenesis is mapping of the transposon self-insertions and, to our best knowledge, there is no publicly available mapping tool designed to analyze such molecular events.

Results

We developed Genome ARTIST, a pairwise gapped aligner tool which works out both issues by means of an original, robust mapping strategy. Genome ARTIST is not d

SUBMITTER: Ecovoiu AA 

PROVIDER: S-EPMC4744444 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature