ABCB19-mediated polar auxin transport modulates Arabidopsis hypocotyl elongation and the endoreplication variant of the cell cycle.

ABSTRACT: Elongation of the Arabidopsis hypocotyl pushes the shoot-producing meristem out of the soil by rapid expansion of cells already present in the embryo. This elongation process is shown here to be impaired by as much as 35% in mutants lacking ABCB19, an ATP-binding cassette membrane protein required for polar auxin transport, during a limited time of fast growth in dim white light beginning 2.5 days after germination. The discovery of high ectopic expression of a cyclin B1;1-based reporter of mitosis throughout abcb19 hypocotyls without an equivalent effect on mitosis prompted investigations of the endoreplication variant of the cell cycle. Flow cytometry performed on nuclei isolated from upper (growing) regions of 3-day-old hypocotyls showed ploidy levels to be lower in abcb19 mutants compa

SUBMITTER: Wu G 

PROVIDER: S-EPMC4744948 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature