Immobilization of Phenylalanine Ammonia-Lyase on Single-Walled Carbon Nanotubes for Stereoselective Biotransformations in Batch and Continuous-Flow Modes.

ABSTRACT: Carboxylated single-walled carbon nanotubes (SwCNT_COOH) were used as a support for the covalent immobilization of phenylalanine ammonia-lyase (PAL) from parsley by two different methods. The nanostructured biocatalysts (SwCNT_COOH-PAL^I and SwCNT_COOH-PAL^II) with low diffusional limitation were tested in the batch-mode kinetic resolution of racemic 2-amino-3-(thiophen-2-yl)propanoic acid (1) to yield a mixture of (R)-1 and (E)-3-(thiophen-2-yl)acrylic acid (2) and in ammonia addition to 2 to yield enantiopure (S)-1. SwCNT_COOH-PAL^II was a stable biocatalyst (>90 % of the original activity remained after six cycles with 1 and after three cycles in 6 m NH

SUBMITTER: Bartha-Vari JH 

PROVIDER: S-EPMC4744988 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature