PrimerMapper: high throughput primer design and graphical assembly for PCR and SNP detection.

ABSTRACT: Primer design represents a widely employed gambit in diverse molecular applications including PCR, sequencing, and probe hybridization. Variations of PCR, including primer walking, allele-specific PCR, and nested PCR provide specialized validation and detection protocols for molecular analyses that often require screening large numbers of DNA fragments. In these cases, automated sequence retrieval and processing become important features, and furthermore, a graphic that provides the user with a visual guide to the distribution of designed primers across targets is most helpful in quickly ascertaining primer coverage. To this end, I describe here, PrimerMapper, which provides a comprehensive graphical user interface that designs robust primers from any number of inputted sequences while pro

SUBMITTER: O'Halloran DM 

PROVIDER: S-EPMC4745053 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature