A genome wide transcriptional model of the complex response to pre-TCR signalling during thymocyte differentiation.

ABSTRACT: Developing thymocytes require pre-TCR signalling to differentiate from CD4-CD8- double negative to CD4+CD8+ double positive cell. Here we followed the transcriptional response to pre-TCR signalling in a synchronised population of differentiating double negative thymocytes. This time series analysis revealed a complex transcriptional response, in which thousands of genes were up and down-regulated before changes in cell surface phenotype were detected. Genome-wide measurement of RNA degradation of individual genes showed great heterogeneity in the rate of degradation between different genes. We therefore used time course expression and degradation data and a genome wide transcriptional modelling (GWTM) strategy to model the transcriptional response of genes up-regulated on pre-TCR signal tr

SUBMITTER: Sahni H 

PROVIDER: S-EPMC4745683 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature