High throughput screening of cytokines, chemokines and matrix metalloproteinases in wound fluid induced by mammary surgery.

ABSTRACT:

Objective

To clarify the composition of wound fluid (WF) and investigate the impact of WF on breast cancer cell lines.

Methods

The proliferation and migration of WF-treated breast cancer cells MDA-MB-231 and MCF-7 were assessed with colony formation test, MTT cell proliferation test and scratch wound test. The quantitative profiles of WF were analyzed using Bio-Plex Pro kits.

Results

The proliferation and migration of WF-treated breast cancer cells were significantly higher than that of untreated cells. Fifteen cytokines, 29 chemokines and 9 matrix metalloproteinases (MMPs) were assessed in WF. The concentrations of these factors were influenced by post-surgery days, neoadjuvant chemotherapy (NAC), TNM stage, pathological type and molecular subtype. The WF harvested f

SUBMITTER: Wang D 

PROVIDER: S-EPMC4745727 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature