Identification and characterization of ANO9 in stage II and III colorectal carcinoma.

ABSTRACT:

Background and objectives

The precise role and potential underlying mechanisms of anoctamin 9 (ANO9) remain largely unknown. This study aims to characterize the role and oncogenic mechanisms of ANO9 in stage II and III colorectal cancer (CRC).

Methods

We examined the expression of ANO9 in colorectal cancerous tissues and cells using real-time quantitative PCR and immunohistochemistry, respectively. Multiple cellular and molecular approaches such as gene transfection, CCK-8 assay, flow cytometry, and invasion assay were also performed to explore its oncogenic mechanisms. Furthermore, the clinical significance of ANO9 in clinical CRC specimens was assessed by clinical correlation and survival analyses.

Results

Lower expression of ANO9 messenger RNA (mRNA) was frequently

SUBMITTER: Li C 

PROVIDER: S-EPMC4745729 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature