Comparing Apples and Oranges?: Next Generation Sequencing and Its Impact on Microbiome Analysis.

ABSTRACT: Rapid advancements in sequencing technologies along with falling costs present widespread opportunities for microbiome studies across a vast and diverse array of environments. These impressive technological developments have been accompanied by a considerable growth in the number of methodological variables, including sampling, storage, DNA extraction, primer pairs, sequencing technology, chemistry version, read length, insert size, and analysis pipelines, amongst others. This increase in variability threatens to compromise both the reproducibility and the comparability of studies conducted. Here we perform the first reported study comparing both amplicon and shotgun sequencing for the three leading next-generation sequencing technologies. These were applied to six human stool samples usin

SUBMITTER: Clooney AG 

PROVIDER: S-EPMC4746063 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature