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Dynamic monitoring of cell mechanical properties using profile microindentation.

ABSTRACT: We have developed a simple and relatively inexpensive system to visualize adherent cells in profile while measuring their mechanical properties using microindentation. The setup allows simultaneous control of cell microenvironment by introducing a micropipette for the delivery of soluble factors or other cell types. We validate this technique against atomic force microscopy measurements and, as a proof of concept, measure the viscoelastic properties of vascular endothelial cells in terms of an apparent stiffness and a dimensionless parameter that describes stress relaxation. Furthermore, we use this technique to monitor the time evolution of these mechanical properties as the cells' actin is depolymerized using cytochalasin-D.

SUBMITTER: Guillou L

PROVIDER: S-EPMC4746699 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Dynamic monitoring of cell mechanical properties using profile microindentation.

Guillou L L Babataheri A A Puech P-H PH Barakat A I AI Husson J J

Scientific reports 20160209

We have developed a simple and relatively inexpensive system to visualize adherent cells in profile while measuring their mechanical properties using microindentation. The setup allows simultaneous control of cell microenvironment by introducing a micropipette for the delivery of soluble factors or other cell types. We validate this technique against atomic force microscopy measurements and, as a proof of concept, measure the viscoelastic properties of vascular endothelial cells in terms of an a ...[more]

PMID: 26857265

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