First report of Y-linked genes in the kissing bug Rhodnius prolixus.

ABSTRACT:

Background

Due to an abundance of repetitive DNA, the annotation of heterochromatic regions of the genome such as the Y chromosome is problematic. The Y chromosome is involved in key biological functions such as male-fertility and sex-determination and hence, accurate identification of its sequences is vital. The hemipteran insect Rhodnius prolixus is an important vector of Chagas disease, a trypanosomiasis affecting 6-7 million people worldwide. Here we report the identification of the first Y-linked genes of this species.

Results

The R. prolixus genome was recently sequenced using separate libraries for each sex and the sequences assembled only with male reads are candidates for Y linkage. We found 766 such candidates and PCR tests with the ten largest ones, confirmed Y-li

SUBMITTER: Koerich LB 

PROVIDER: S-EPMC4746886 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature