Whole-Exome Sequencing Identifies Novel Somatic Mutations in Chinese Breast Cancer Patients.

ABSTRACT: Most breast cancer genomes harbor complex mutational landscapes. Somatic alterations have been predominantly discovered in breast cancer patients of European ancestry; however, little is known about somatic aberration in patients of other ethnic groups including Asians. In the present study, whole-exome sequencing (WES) was conducted in DNA extracted from tumor and matched adjacent normal tissue samples from eleven early onset breast cancer patients who were included in the Shanghai Breast Cancer Study. We discovered 159 somatic missense and ten nonsense mutations distributed among 167 genes. The most frequent 50 somatic mutations identified by WES were selected for validation using Sequenom MassARRAY system in the eleven breast cancer patients and an additional 433 tumor and 921 normal ti

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC4747093 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature