Personalized comprehensive molecular profiling of high risk osteosarcoma: Implications and limitations for precision medicine.

ABSTRACT:

Background

Despite advances in molecular medicine over recent decades, there has been little advancement in the treatment of osteosarcoma. We performed comprehensive molecular profiling in two cases of metastatic and chemotherapy-refractory osteosarcoma to guide molecularly targeted therapy.

Patients and methods

Hybridization capture of >300 cancer-related genes plus introns from 28 genes often rearranged or altered in cancer was applied to >50 ng of DNA extracted from tumor samples from two patients with recurrent, metastatic osteosarcoma. The DNA from each sample was sequenced to high, uniform coverage. Immunohistochemical probes and morphoproteomics analysis were performed, in addition to fluorescence in situ hybridization. All analyses were performed in CLIA-certified la

SUBMITTER: Subbiah V 

PROVIDER: S-EPMC4747358 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature