Whole genome sequence analysis of BT-474 using complete Genomics' standard and long fragment read technologies.

ABSTRACT:

Background

The cell line BT-474 is a popular cell line for studying the biology of cancer and developing novel drugs. However, there is no complete, published genome sequence for this highly utilized scientific resource. In this study we sought to provide a comprehensive and useful data set for the scientific community by generating a whole genome sequence for BT-474.

Findings

Five μg of genomic DNA, isolated from an early passage of the BT-474 cell line, was used to generate a whole genome sequence (114X coverage) using Complete Genomics' standard sequencing process. To provide additional variant phasing and structural variation data we also processed and analyzed two separate libraries of 5 and 6 individual cells to depths of 99X and 87X, respectively, using Complete Genom

SUBMITTER: Ciotlos S 

PROVIDER: S-EPMC4748558 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature