High content screening application for cell-type specific behaviour in heterogeneous primary breast epithelial subpopulations.

ABSTRACT:

Background

The complex interaction between multiple cell types and the microenvironment underlies the diverse pathways to carcinogenesis and necessitates sophisticated approaches to in vitro hypotheses testing. The combination of mixed culture format with high content immunofluorescence screening technology provides a powerful platform for observation of cell type specific behavior.

Methods

We have developed a versatile, high-throughput method for assessing cell-type specific responses. In addition to the specificity and sensitivity offered traditionally by immunofluorescent detection in flow cytometry, the 'in-cell' analysis method we describe provides the added benefits of higher throughput and the ability to analyse protein subcellular localisation in situ. Furthermore, e

SUBMITTER: Johnston RL 

PROVIDER: S-EPMC4748588 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature