Antibody-like Biorecognition Sites for Proteins from Surface Imprinting on Nanoparticles.

ABSTRACT: Natural antibodies are used widely for important applications such as biomedical analysis, cancer therapy, and directed drug delivery, but they are expensive and may have limited stability. This study describes synthesis of antibody-like binding sites by molecular imprinting on silica nanoparticles (SiNP) using a combination of four organosilane monomers with amino acid-like side chains providing hydrophobic, hydrophilic, and H-bonding interactions with target proteins. This approach provided artificial antibody (AA) nanoparticles with good selectivity and specificity to binding domains on target proteins in a relatively low-cost synthesis. The AAs were made by polymer grafting onto SiNPs for human serum albumin (HSA) and glucose oxidase (GOx). Binding affinity, selectivity, and specificit

SUBMITTER: Bhakta S 

PROVIDER: S-EPMC4749148 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature