Human Liver Infection in a Dish: Easy-To-Build 3D Liver Models for Studying Microbial Infection.

ABSTRACT: Human liver infection is a major cause of death worldwide, but fundamental studies on infectious diseases affecting humans have been hampered by the lack of robust experimental models that accurately reproduce pathogen-host interactions in an environment relevant for the human disease. In the case of liver infection, one consequence of this absence of relevant models is a lack of understanding of how pathogens cross the sinusoidal endothelial barrier and parenchyma. To fill that gap we elaborated human 3D liver in vitro models, composed of human liver sinusoidal endothelial cells (LSEC) and Huh-7 hepatoma cells as hepatocyte model, layered in a structure mimicking the hepatic sinusoid, which enable studies of key features of early steps of hepatic infection. Built with established cell lin

SUBMITTER: Petropolis DB 

PROVIDER: S-EPMC4749187 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature