A Lab Assembled Microcontroller-Based Sensor Module for Continuous Oxygen Measurement in Portable Hypoxia Chambers.

ABSTRACT:

Background

Hypoxia-based cell culture experiments are routine and essential components of in vitro cancer research. Most laboratories use low-cost portable modular chambers to achieve hypoxic conditions for cell cultures, where the sealed chambers are purged with a gas mixture of preset O2 concentration. Studies are conducted under the assumption that hypoxia remains unaltered throughout the 48 to 72 hour duration of such experiments. Since these chambers lack any sensor or detection system to monitor gas-phase O2, the cell-based data tend to be non-uniform due to the ad hoc nature of the experimental setup.

Methodology

With the availability of low-cost open-source microcontroller-based electronic project kits, it is now possible for researchers to program these with easy-to

SUBMITTER: Mathupala SP 

PROVIDER: S-EPMC4749204 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature