Protein Expression Profiling of Giant Cell Tumors of Bone Treated with Denosumab.

ABSTRACT: Giant cell tumors of bone (GCTB) are locally aggressive osteolytic bone tumors. Recently, some clinical trials have shown that denosumab is a novel and effective therapeutic option for aggressive and recurrent GCTB. This study was performed to investigate the molecular mechanism underlying the therapeutic effect of denosumab. Comparative proteomic analyses were performed using GCTB samples which were taken before and after denosumab treatment. Each expression profile was analyzed using the software program to further understand the affected biological network. One of identified proteins was further evaluated by gelatin zymography and an immunohistochemical analysis. We identified 13 consistently upregulated proteins and 19 consistently downregulated proteins in the pre- and post-denosumab

SUBMITTER: Mukaihara K 

PROVIDER: S-EPMC4749282 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature